Researchers Develop 3D-Printed Liver Lobules for Drug Screening andRegeneration

Researchers at Nanjing University developed 3D-printed biomimetic liver lobules with vascularized channels for drug screening and liver regeneration. The engineered tissues were validated for drug screening and promoted liver regeneration when transplanted into rats with acute liver failure.

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Aqsa Younas Rana
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Researchers Develop 3D-Printed Liver Lobules for Drug Screening andRegeneration

Researchers Develop 3D-Printed Liver Lobules for Drug Screening andRegeneration

A team of researchers led by Prof. Yuanjin Zhao at Nanjing University in China has successfully developed 3D-printed biomimetic liver lobules with vascularized channels. These engineered liver tissues can be used for drug screening and have the potential to promote liver regeneration.

Why this matters: This breakthrough technology has the potential to revolutionize the field of tissue engineering and regenerative medicine, leading to improved treatment options for patients with liver diseases. Moreover, the development of 3D-printed liver lobules could also accelerate the discovery of new drugs and reduce the need for animal testing.

The researchers utilized spatial proteogenomic datasets to identify the multicellular composition of the normal human liver. They then employed a novel 3D printing strategy to print and carve liver cell sheets with hexagonal hollow cross-sectional structures. By stacking multiple layers of these lobule structures, the team was able to mimic the microscale units of the liver.

To create vascularized channels within the bioengineered liver lobules, the researchers infiltrated endothelial cells into the hollow cross-sections of the assembled lobule structures. This allowed for nutrient diffusion and drug perfusion throughout the tissue constructs.

The vascularized liver lobules were integrated with microfluidics to construct liver chips, which were validated for their application indrug screening. Furthermore, the researchers assembled the bioengineered liver lobules into larger physiological structures. When transplanted into rats with acute liver failure, these engineered tissues demonstrated the ability to promote liver regeneration.

The study, titled"Biomimetic hepatic lobules from three-dimensional imprinted cell sheets,"was published in the journal Science Bulletin on May 10, 2024. The development of these 3D-printed liver lobules with vascularized channels represents a significant step forward in the field of tissue engineering and regenerative medicine. This breakthrough technology holds promise for improving drug screening methods and providing new treatment options for patients with liver diseases.